hec 1 a immortalized human epithelial endometrium cells Search Results


human endometrial epithelial cell line hec1a  (ATCC)
96
ATCC human endometrial epithelial cell line hec1a
Figure 6. WNK1 ablation led to FOXO1 nuclear exclusion via AKT phosphorylation, which was associated with decreased PP2A phosphatase expres- sion. (A) Western blot showing levels of phosphorylated and total AKT and FOXO1 in <t>HEC1A</t> cells and THESCs transfected with 48 nM small interfering control (siCTRL) or siWNK1. (B) Immunofluorescence showing FOXO1 subcellular localization (green), with nuclei presented in DAPI in HEC1A and THESC control cells (columns 1, 4), siWNK1-transfected cells (columns 2, 5), and GDC0941-treated, siWNK1-transfected cells (columns 3, 6); scale bars: 20 μm. (C) Expression of FOXO1, AKT, and PI3K members in HEC1A cells and THESCs transfected with siCTRL or siWNK1 and treated with AKT inhibitor GDC0941. (D and E) Expression of PI3K proteins (D) and MTOR, PP2A subunits, and PTEN (E) in Wnk1fl/fl and Wnk1d/d uteri on PPD 4.5.
Human Endometrial Epithelial Cell Line Hec1a, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human endometrial epithelial cell lines  (ATCC)
96
ATCC human endometrial epithelial cell lines
Figure 6. WNK1 ablation led to FOXO1 nuclear exclusion via AKT phosphorylation, which was associated with decreased PP2A phosphatase expres- sion. (A) Western blot showing levels of phosphorylated and total AKT and FOXO1 in <t>HEC1A</t> cells and THESCs transfected with 48 nM small interfering control (siCTRL) or siWNK1. (B) Immunofluorescence showing FOXO1 subcellular localization (green), with nuclei presented in DAPI in HEC1A and THESC control cells (columns 1, 4), siWNK1-transfected cells (columns 2, 5), and GDC0941-treated, siWNK1-transfected cells (columns 3, 6); scale bars: 20 μm. (C) Expression of FOXO1, AKT, and PI3K members in HEC1A cells and THESCs transfected with siCTRL or siWNK1 and treated with AKT inhibitor GDC0941. (D and E) Expression of PI3K proteins (D) and MTOR, PP2A subunits, and PTEN (E) in Wnk1fl/fl and Wnk1d/d uteri on PPD 4.5.
Human Endometrial Epithelial Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hec 1a endometrial epithelial cells  (ATCC)
96
ATCC hec 1a endometrial epithelial cells
Figure 6. WNK1 ablation led to FOXO1 nuclear exclusion via AKT phosphorylation, which was associated with decreased PP2A phosphatase expres- sion. (A) Western blot showing levels of phosphorylated and total AKT and FOXO1 in <t>HEC1A</t> cells and THESCs transfected with 48 nM small interfering control (siCTRL) or siWNK1. (B) Immunofluorescence showing FOXO1 subcellular localization (green), with nuclei presented in DAPI in HEC1A and THESC control cells (columns 1, 4), siWNK1-transfected cells (columns 2, 5), and GDC0941-treated, siWNK1-transfected cells (columns 3, 6); scale bars: 20 μm. (C) Expression of FOXO1, AKT, and PI3K members in HEC1A cells and THESCs transfected with siCTRL or siWNK1 and treated with AKT inhibitor GDC0941. (D and E) Expression of PI3K proteins (D) and MTOR, PP2A subunits, and PTEN (E) in Wnk1fl/fl and Wnk1d/d uteri on PPD 4.5.
Hec 1a Endometrial Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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i e an3  (ATCC)
96
ATCC i e an3
Figure 6. WNK1 ablation led to FOXO1 nuclear exclusion via AKT phosphorylation, which was associated with decreased PP2A phosphatase expres- sion. (A) Western blot showing levels of phosphorylated and total AKT and FOXO1 in <t>HEC1A</t> cells and THESCs transfected with 48 nM small interfering control (siCTRL) or siWNK1. (B) Immunofluorescence showing FOXO1 subcellular localization (green), with nuclei presented in DAPI in HEC1A and THESC control cells (columns 1, 4), siWNK1-transfected cells (columns 2, 5), and GDC0941-treated, siWNK1-transfected cells (columns 3, 6); scale bars: 20 μm. (C) Expression of FOXO1, AKT, and PI3K members in HEC1A cells and THESCs transfected with siCTRL or siWNK1 and treated with AKT inhibitor GDC0941. (D and E) Expression of PI3K proteins (D) and MTOR, PP2A subunits, and PTEN (E) in Wnk1fl/fl and Wnk1d/d uteri on PPD 4.5.
I E An3, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hec 1a  (ATCC)
96
ATCC hec 1a
Figure 6. WNK1 ablation led to FOXO1 nuclear exclusion via AKT phosphorylation, which was associated with decreased PP2A phosphatase expres- sion. (A) Western blot showing levels of phosphorylated and total AKT and FOXO1 in <t>HEC1A</t> cells and THESCs transfected with 48 nM small interfering control (siCTRL) or siWNK1. (B) Immunofluorescence showing FOXO1 subcellular localization (green), with nuclei presented in DAPI in HEC1A and THESC control cells (columns 1, 4), siWNK1-transfected cells (columns 2, 5), and GDC0941-treated, siWNK1-transfected cells (columns 3, 6); scale bars: 20 μm. (C) Expression of FOXO1, AKT, and PI3K members in HEC1A cells and THESCs transfected with siCTRL or siWNK1 and treated with AKT inhibitor GDC0941. (D and E) Expression of PI3K proteins (D) and MTOR, PP2A subunits, and PTEN (E) in Wnk1fl/fl and Wnk1d/d uteri on PPD 4.5.
Hec 1a, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hec-1a (human endometrial epithelial) cells  (BioWhittaker Molecular Applications)
90
BioWhittaker Molecular Applications hec-1a (human endometrial epithelial) cells
Figure 6. WNK1 ablation led to FOXO1 nuclear exclusion via AKT phosphorylation, which was associated with decreased PP2A phosphatase expres- sion. (A) Western blot showing levels of phosphorylated and total AKT and FOXO1 in <t>HEC1A</t> cells and THESCs transfected with 48 nM small interfering control (siCTRL) or siWNK1. (B) Immunofluorescence showing FOXO1 subcellular localization (green), with nuclei presented in DAPI in HEC1A and THESC control cells (columns 1, 4), siWNK1-transfected cells (columns 2, 5), and GDC0941-treated, siWNK1-transfected cells (columns 3, 6); scale bars: 20 μm. (C) Expression of FOXO1, AKT, and PI3K members in HEC1A cells and THESCs transfected with siCTRL or siWNK1 and treated with AKT inhibitor GDC0941. (D and E) Expression of PI3K proteins (D) and MTOR, PP2A subunits, and PTEN (E) in Wnk1fl/fl and Wnk1d/d uteri on PPD 4.5.
Hec 1a (Human Endometrial Epithelial) Cells, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kle  (CH Instruments)
90
CH Instruments kle
Figure 6. WNK1 ablation led to FOXO1 nuclear exclusion via AKT phosphorylation, which was associated with decreased PP2A phosphatase expres- sion. (A) Western blot showing levels of phosphorylated and total AKT and FOXO1 in <t>HEC1A</t> cells and THESCs transfected with 48 nM small interfering control (siCTRL) or siWNK1. (B) Immunofluorescence showing FOXO1 subcellular localization (green), with nuclei presented in DAPI in HEC1A and THESC control cells (columns 1, 4), siWNK1-transfected cells (columns 2, 5), and GDC0941-treated, siWNK1-transfected cells (columns 3, 6); scale bars: 20 μm. (C) Expression of FOXO1, AKT, and PI3K members in HEC1A cells and THESCs transfected with siCTRL or siWNK1 and treated with AKT inhibitor GDC0941. (D and E) Expression of PI3K proteins (D) and MTOR, PP2A subunits, and PTEN (E) in Wnk1fl/fl and Wnk1d/d uteri on PPD 4.5.
Kle, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mccoy’s 5a medium  (Biochrom)
90
Biochrom mccoy’s 5a medium
Figure 6. WNK1 ablation led to FOXO1 nuclear exclusion via AKT phosphorylation, which was associated with decreased PP2A phosphatase expres- sion. (A) Western blot showing levels of phosphorylated and total AKT and FOXO1 in <t>HEC1A</t> cells and THESCs transfected with 48 nM small interfering control (siCTRL) or siWNK1. (B) Immunofluorescence showing FOXO1 subcellular localization (green), with nuclei presented in DAPI in HEC1A and THESC control cells (columns 1, 4), siWNK1-transfected cells (columns 2, 5), and GDC0941-treated, siWNK1-transfected cells (columns 3, 6); scale bars: 20 μm. (C) Expression of FOXO1, AKT, and PI3K members in HEC1A cells and THESCs transfected with siCTRL or siWNK1 and treated with AKT inhibitor GDC0941. (D and E) Expression of PI3K proteins (D) and MTOR, PP2A subunits, and PTEN (E) in Wnk1fl/fl and Wnk1d/d uteri on PPD 4.5.
Mccoy’s 5a Medium, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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colo 205  (ATCC)
96
ATCC colo 205
Figure 6. WNK1 ablation led to FOXO1 nuclear exclusion via AKT phosphorylation, which was associated with decreased PP2A phosphatase expres- sion. (A) Western blot showing levels of phosphorylated and total AKT and FOXO1 in <t>HEC1A</t> cells and THESCs transfected with 48 nM small interfering control (siCTRL) or siWNK1. (B) Immunofluorescence showing FOXO1 subcellular localization (green), with nuclei presented in DAPI in HEC1A and THESC control cells (columns 1, 4), siWNK1-transfected cells (columns 2, 5), and GDC0941-treated, siWNK1-transfected cells (columns 3, 6); scale bars: 20 μm. (C) Expression of FOXO1, AKT, and PI3K members in HEC1A cells and THESCs transfected with siCTRL or siWNK1 and treated with AKT inhibitor GDC0941. (D and E) Expression of PI3K proteins (D) and MTOR, PP2A subunits, and PTEN (E) in Wnk1fl/fl and Wnk1d/d uteri on PPD 4.5.
Colo 205, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jar  (ATCC)
95
ATCC jar
Figure 6. WNK1 ablation led to FOXO1 nuclear exclusion via AKT phosphorylation, which was associated with decreased PP2A phosphatase expres- sion. (A) Western blot showing levels of phosphorylated and total AKT and FOXO1 in <t>HEC1A</t> cells and THESCs transfected with 48 nM small interfering control (siCTRL) or siWNK1. (B) Immunofluorescence showing FOXO1 subcellular localization (green), with nuclei presented in DAPI in HEC1A and THESC control cells (columns 1, 4), siWNK1-transfected cells (columns 2, 5), and GDC0941-treated, siWNK1-transfected cells (columns 3, 6); scale bars: 20 μm. (C) Expression of FOXO1, AKT, and PI3K members in HEC1A cells and THESCs transfected with siCTRL or siWNK1 and treated with AKT inhibitor GDC0941. (D and E) Expression of PI3K proteins (D) and MTOR, PP2A subunits, and PTEN (E) in Wnk1fl/fl and Wnk1d/d uteri on PPD 4.5.
Jar, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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thp-1  (ATCC)
99
ATCC thp-1
Figure 6. WNK1 ablation led to FOXO1 nuclear exclusion via AKT phosphorylation, which was associated with decreased PP2A phosphatase expres- sion. (A) Western blot showing levels of phosphorylated and total AKT and FOXO1 in <t>HEC1A</t> cells and THESCs transfected with 48 nM small interfering control (siCTRL) or siWNK1. (B) Immunofluorescence showing FOXO1 subcellular localization (green), with nuclei presented in DAPI in HEC1A and THESC control cells (columns 1, 4), siWNK1-transfected cells (columns 2, 5), and GDC0941-treated, siWNK1-transfected cells (columns 3, 6); scale bars: 20 μm. (C) Expression of FOXO1, AKT, and PI3K members in HEC1A cells and THESCs transfected with siCTRL or siWNK1 and treated with AKT inhibitor GDC0941. (D and E) Expression of PI3K proteins (D) and MTOR, PP2A subunits, and PTEN (E) in Wnk1fl/fl and Wnk1d/d uteri on PPD 4.5.
Thp 1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ht-29  (ATCC)
99
ATCC ht-29
Figure 6. WNK1 ablation led to FOXO1 nuclear exclusion via AKT phosphorylation, which was associated with decreased PP2A phosphatase expres- sion. (A) Western blot showing levels of phosphorylated and total AKT and FOXO1 in <t>HEC1A</t> cells and THESCs transfected with 48 nM small interfering control (siCTRL) or siWNK1. (B) Immunofluorescence showing FOXO1 subcellular localization (green), with nuclei presented in DAPI in HEC1A and THESC control cells (columns 1, 4), siWNK1-transfected cells (columns 2, 5), and GDC0941-treated, siWNK1-transfected cells (columns 3, 6); scale bars: 20 μm. (C) Expression of FOXO1, AKT, and PI3K members in HEC1A cells and THESCs transfected with siCTRL or siWNK1 and treated with AKT inhibitor GDC0941. (D and E) Expression of PI3K proteins (D) and MTOR, PP2A subunits, and PTEN (E) in Wnk1fl/fl and Wnk1d/d uteri on PPD 4.5.
Ht 29, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 6. WNK1 ablation led to FOXO1 nuclear exclusion via AKT phosphorylation, which was associated with decreased PP2A phosphatase expres- sion. (A) Western blot showing levels of phosphorylated and total AKT and FOXO1 in HEC1A cells and THESCs transfected with 48 nM small interfering control (siCTRL) or siWNK1. (B) Immunofluorescence showing FOXO1 subcellular localization (green), with nuclei presented in DAPI in HEC1A and THESC control cells (columns 1, 4), siWNK1-transfected cells (columns 2, 5), and GDC0941-treated, siWNK1-transfected cells (columns 3, 6); scale bars: 20 μm. (C) Expression of FOXO1, AKT, and PI3K members in HEC1A cells and THESCs transfected with siCTRL or siWNK1 and treated with AKT inhibitor GDC0941. (D and E) Expression of PI3K proteins (D) and MTOR, PP2A subunits, and PTEN (E) in Wnk1fl/fl and Wnk1d/d uteri on PPD 4.5.

Journal: JCI insight

Article Title: WNK1 regulates uterine homeostasis and its ability to support pregnancy.

doi: 10.1172/jci.insight.141832

Figure Lengend Snippet: Figure 6. WNK1 ablation led to FOXO1 nuclear exclusion via AKT phosphorylation, which was associated with decreased PP2A phosphatase expres- sion. (A) Western blot showing levels of phosphorylated and total AKT and FOXO1 in HEC1A cells and THESCs transfected with 48 nM small interfering control (siCTRL) or siWNK1. (B) Immunofluorescence showing FOXO1 subcellular localization (green), with nuclei presented in DAPI in HEC1A and THESC control cells (columns 1, 4), siWNK1-transfected cells (columns 2, 5), and GDC0941-treated, siWNK1-transfected cells (columns 3, 6); scale bars: 20 μm. (C) Expression of FOXO1, AKT, and PI3K members in HEC1A cells and THESCs transfected with siCTRL or siWNK1 and treated with AKT inhibitor GDC0941. (D and E) Expression of PI3K proteins (D) and MTOR, PP2A subunits, and PTEN (E) in Wnk1fl/fl and Wnk1d/d uteri on PPD 4.5.

Article Snippet: Human endometrial epithelial cell line HEC1A and telomerase-THESCs were obtained from American Type Culture Collection (Rockville, Maryland, USA).

Techniques: Phospho-proteomics, Western Blot, Transfection, Control, Immunofluorescence, Expressing

Figure 7. WNK1 regulated AKT signaling through direct interaction with PPP2R1A. (A) WNK1 and YFP expression in HEC1A cells transfected with the YFP-expressing control plasmid (cYFP) or YFP-tagged WNK1 expression construct (c4161). (B) Coimmunoprecipitation of WNK1 and PPP2R1A with YFP from HEC1A whole cell lysate, as indicated by Western blotting. (C) Expression of PPP2R1A and PP2A subunits in HEC1A cells transfected with 24 nM siCTRL or siWNK1 for 72 hours. (D) Expression of PP2A subunits A, B, and C; AKT; and FOXO1 in HEC1A cells transfected with 72 nM siCTRL or siPPP2R1A for 72 hours. (E) Diagram illustrating the WNK1/PP2A/AKT/FOXO1 signaling axis. WNK1 physically interacts with PPP2R1A, the alpha isoform of the scaffold subunit that forms the functional PP2A subunit. PP2A negatively regulates AKT, and AKT negatively regulates FOXO1 by phosphorylation and nuclear exclusion. AKT also promotes epithelial cell proliferation. As indicated by the blue and red arrows, decreased or loss of WNK1 will then lead to decreased PP2A activity, AKT hypersignaling, and increased cytoplasmic FOXO1 retention and epithelial proliferation.

Journal: JCI insight

Article Title: WNK1 regulates uterine homeostasis and its ability to support pregnancy.

doi: 10.1172/jci.insight.141832

Figure Lengend Snippet: Figure 7. WNK1 regulated AKT signaling through direct interaction with PPP2R1A. (A) WNK1 and YFP expression in HEC1A cells transfected with the YFP-expressing control plasmid (cYFP) or YFP-tagged WNK1 expression construct (c4161). (B) Coimmunoprecipitation of WNK1 and PPP2R1A with YFP from HEC1A whole cell lysate, as indicated by Western blotting. (C) Expression of PPP2R1A and PP2A subunits in HEC1A cells transfected with 24 nM siCTRL or siWNK1 for 72 hours. (D) Expression of PP2A subunits A, B, and C; AKT; and FOXO1 in HEC1A cells transfected with 72 nM siCTRL or siPPP2R1A for 72 hours. (E) Diagram illustrating the WNK1/PP2A/AKT/FOXO1 signaling axis. WNK1 physically interacts with PPP2R1A, the alpha isoform of the scaffold subunit that forms the functional PP2A subunit. PP2A negatively regulates AKT, and AKT negatively regulates FOXO1 by phosphorylation and nuclear exclusion. AKT also promotes epithelial cell proliferation. As indicated by the blue and red arrows, decreased or loss of WNK1 will then lead to decreased PP2A activity, AKT hypersignaling, and increased cytoplasmic FOXO1 retention and epithelial proliferation.

Article Snippet: Human endometrial epithelial cell line HEC1A and telomerase-THESCs were obtained from American Type Culture Collection (Rockville, Maryland, USA).

Techniques: Expressing, Transfection, Control, Plasmid Preparation, Construct, Western Blot, Functional Assay, Phospho-proteomics, Activity Assay